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Fig. 4 EGFP-labeled cells co-express myeloid lineage markers. Dual immunohistological detection of EGFP and in situ hybridization detection of lysC (A and B), L-plastin (C and D) within 48 hpf and fms (E), mpo (F) within 32 hpf lysC::EGFP animals. (A/C/E/F and B/D) Summed Z stacks within posterior ICM compartment and within hindbrain, respectively, of transgenic animals. Arrow in C and E denotes EGFP-negative cell expressing L-plastin and fms, respectively. Arrow in F denotes EGFP-expressing cell that does not co-express mpo. Anterior to left in all images. Scale bars: 50 μm in A; 10 μm in B-F.
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