Analysis of hematopoietic and endothelial expansion in clo in response to forced expression of scl. Double ISH (A–D, I–P) using myoD in red and gata1, gata2, or lmo2 in blue, or single ISH using flk1 (E–H) or lmo2 (Q–T), in wild-type and clo-/- embryos injected with scl RNA (+scl) or uninjected controls, as indicated. In G, H, brackets indicate anterior endothelial cells, and arrows indicate posterior lateral mesoderm. In I and J, arrowhead indicates expression of flk1 in the tail bud. Embryos are between the 10- and 12-somite stage (A–P) or the 20 somite stage (Q–T).
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