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Fig. 9 Effect of early Fgf inhibitor treatment on erm, prdm1, fgf24 and tbx5 expression. Whole-mount in situ hybridization on 20.5 hpf (23-somite stage) (A,B,E,F,I,J,M,N) and 24 hpf (C,D,G,H,K,L,O,P) control DMSO and SU5402-treated embryos. Probes are as indicated in each panel. (A-D) erm expression is abolished in SU5402-treated embryos. (E-H) prdm1 expression is downregulated in SU5402-treated embryos. (I-L) Upon SU5402 treatment, Fgf24 is still present at 20.5 hpf but becomes strongly reduced at 24 hpf. (M-P) tbx5 expression is not affected at the 23-somite stage (M,N) but is reduced in 24 hpf embryos upon SU5402 treatment (O,P).