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Fig. 3 Temporal expression profiles of the zebrafish group B1 sox genes determined by reverse transcriptase-polymerase chain reaction (RT-PCR). Total RNA isolates were prepared from embryos at six different stages (32 cells, 1,000 cells, 30% epiboly, tail bud, 24 hr, and 48 hr) and subjected to RT-PCR analysis. Both β-actin and ef1 were used as controls. No PCR products were detected when reverse transcriptase was omitted from the reaction (data not shown).
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