Gene Trap Construct

Gt(GBT-B1)

ID

ZDB-GTCONSTRCT-131119-1

Name

Gt(GBT-B1)

Previous Names

Gt(LOXP-FRT-14xUAS:EGFP-FRT,GAL4-VP16-LOXP)

Type

engineered_plasmid

Regulatory Regions

UAS (1)

Coding Sequences

EGFP (1)
GAL4 (1)

[Em λ][Ex λ]

Fpbase:EGFP

Contains Sequences

FRT (1)
LOXP (1)
TOL2 (1)
VP16 (1)

Note

This construct is based on GBT-R15, with AUG-less mRFP replaced by AUG-less Gal4-VP16. For direct detection of gene trap events, GBT-B1 also contains a UAS:eGFP cassette.

Plasmid Map

None

Genomic Features

That Utilize Gt(GBT-B1) Construct

Genomic Feature	Affected Genomic Regions
tpl10Gt	atp1a3a
tpl11Gt	bbs7
tpl12Gt	si:dkey-24m12.2
tpl13Gt	si:dkey-9i23.6
tpl14Gt	osbpl10b
tpl15Gt	eef1a1l2
tpl16Gt	ebf3a
tpl17Gt	plecb
tpl18Gt	tent5bb
tpl19Gt	ift70
tpl20Gt	lasp1
tpl21Gt	triqk
tpl22Gt	baiap2l1a
tpl23Gt	fnbp1b
tpl24Gt	cyp26c1
tpl25Gt	srp68
tpl26Gt	fam89a
tpl27Gt	snap25b
tpl28Gt
tpl29Gt
tpl30Gt
tpl31Gt
tpl32Gt
tpl34Gt
tpl35Gt
tpl36Gt
tpl37Gt
tpl38Gt
tpl39Gt
tpl3Gt	nudc
tpl40Gt
tpl41Gt
tpl42Gt
tpl43Gt
tpl4Gt	stat5a
tpl5Gt	st6galnac5a
tpl6Gt	nsfa
tpl7Gt	jam3b
tpl8Gt	zfp36l1a
tpl9Gt	nfe2l1b

Transgenics

That Utilize Gt(GBT-B1) Construct

Fish	Affected Genomic Regions	Phenotype	Gene Expression
atp1a3a^tpl10Gt/+; tpl2Tg/+ (AB)	atp1a3a
atp1a3a^tpl10Gt; tpl2Tg	atp1a3a		2 figures from 2 publications
baiap2l1a^tpl22Gt/+; tpl2Tg/+ (AB)	baiap2l1a
baiap2l1a^tpl22Gt; tpl2Tg	baiap2l1a		2 figures from 2 publications
bbs7^tpl11Gt/+; tpl2Tg/+ (AB)	bbs7
bbs7^tpl11Gt; tpl2Tg	bbs7		2 figures from 2 publications
cyp26c1^tpl24Gt/+; tpl2Tg/+ (AB)	cyp26c1
cyp26c1^tpl24Gt; tpl2Tg	cyp26c1		3 figures from 2 publications
ebf3a^tpl16Gt/+; tpl2Tg/+ (AB)	ebf3a
ebf3a^tpl16Gt; tpl2Tg	ebf3a		3 figures from 2 publications
eef1a1l2^tpl15Gt; tpl2Tg	eef1a1l2		Fig. 3 from Balciuniene et al., 2013
fam89a^tpl26Gt/+; tpl2Tg/+ (AB)	fam89a
fam89a^tpl26Gt; tpl2Tg	fam89a		2 figures from 2 publications
fnbp1b^tpl23Gt/+; tpl2Tg/+ (AB)	fnbp1b
fnbp1b^tpl23Gt; tpl2Tg	fnbp1b		2 figures from 2 publications
ift70^tpl19Gt/+; tpl2Tg/+ (AB)	ift70
ift70^tpl19Gt; tpl2Tg	ift70		2 figures from 2 publications
jam3b^tpl7Gt/+; tpl2Tg/+ (AB)	jam3b
jam3b^tpl7Gt; tpl2Tg	jam3b		2 figures from 2 publications
lasp1^tpl20Gt/+; tpl2Tg/+ (AB)	lasp1
lasp1^tpl20Gt; tpl2Tg	lasp1		2 figures from 2 publications
nfe2l1b^tpl9Gt/+; tpl2Tg/+ (AB)	nfe2l1b
nfe2l1b^tpl9Gt; tpl2Tg	nfe2l1b		2 figures from 2 publications
nsfa^tpl6Gt/+	nsfa	Fig. 1 from Malafoglia et al., 2014	Fig. 1 from Malafoglia et al., 2014
nsfa^tpl6Gt/+; tpl2Tg/+ (AB)	nsfa
nsfa^tpl6Gt; tpl131Tg (AB)	nsfa
nsfa^tpl6Gt; tpl131Tg + MO1-dnm1a (AB)		Table 2 from Bragato et al., 2023
nsfa^tpl6Gt; tpl131Tg + MO2-dnm1a (AB)		Table 2 from Bragato et al., 2023
nsfa^tpl6Gt; tpl2Tg	nsfa		2 figures from 2 publications
nudc^tpl3Gt/+; tpl2Tg/+ (AB)	nudc
nudc^tpl3Gt; tpl2Tg	nudc		1 figure from Balciuniene et al., 2013
osbpl10b^tpl14Gt/+; tpl2Tg/+ (AB)	osbpl10b
osbpl10b^tpl14Gt; tpl2Tg	osbpl10b		2 figures from 2 publications
plecb^tpl17Gt/+; tpl2Tg/+ (AB)	plecb
plecb^tpl17Gt; tpl2Tg	plecb		2 figures from 2 publications
si:dkey-24m12.2^tpl12Gt/+; tpl2Tg/+ (AB)	si:dkey-24m12.2
si:dkey-24m12.2^tpl12Gt; tpl2Tg	si:dkey-24m12.2		2 figures from 2 publications
si:dkey-9i23.6^tpl13Gt/+; tpl2Tg/+ (AB)	si:dkey-9i23.6
si:dkey-9i23.6^tpl13Gt; tpl2Tg	si:dkey-9i23.6		2 figures from 2 publications
snap25b^tpl27Gt/+; tpl2Tg/+ (AB)	snap25b
snap25b^tpl27Gt; tpl2Tg	snap25b		3 figures from 2 publications
srp68^tpl25Gt/+; tpl2Tg/+ (AB)	srp68
srp68^tpl25Gt; tpl2Tg	srp68		2 figures from 2 publications
st6galnac5a^tpl5Gt/+; tpl2Tg/+ (AB)	st6galnac5a
st6galnac5a^tpl5Gt; tpl2Tg	st6galnac5a		2 figures from 2 publications
stat5a^tpl4Gt/+; tpl2Tg/+ (AB)	stat5a
stat5a^tpl4Gt; tpl2Tg	stat5a		2 figures from 2 publications
tent5bb^tpl18Gt/+; tpl2Tg/+ (AB)	tent5bb
tent5bb^tpl18Gt; tpl2Tg	tent5bb		2 figures from 2 publications
tpl28Gt/+; tpl2Tg/+ (AB)
tpl28Gt; tpl2Tg			2 figures from 2 publications
tpl29Gt/+; tpl2Tg/+ (AB)
tpl29Gt; tpl2Tg			2 figures from 2 publications
tpl2Tg/+; tpl30Gt/+ (AB)
tpl2Tg/+; tpl31Gt/+ (AB)
tpl2Tg/+; tpl32Gt/+ (AB)
tpl2Tg/+; tpl34Gt/+ (AB)
tpl2Tg/+; tpl35Gt/+ (AB)
tpl2Tg/+; tpl36Gt/+ (AB)
tpl2Tg/+; tpl37Gt/+ (AB)
tpl2Tg/+; tpl38Gt/+ (AB)
tpl2Tg/+; tpl39Gt/+ (AB)
tpl2Tg/+; tpl40Gt/+ (AB)
tpl2Tg/+; tpl41Gt/+ (AB)
tpl2Tg/+; tpl42Gt/+ (AB)
tpl2Tg/+; tpl43Gt/+ (AB)
tpl2Tg; tpl30Gt			2 figures from 2 publications
tpl2Tg; tpl31Gt			2 figures from 2 publications
tpl2Tg; tpl32Gt			2 figures from 2 publications
tpl2Tg; tpl34Gt			2 figures from 2 publications
tpl2Tg; tpl35Gt			2 figures from 2 publications
tpl2Tg; tpl36Gt			2 figures from 2 publications
tpl2Tg; tpl37Gt			2 figures from 2 publications
tpl2Tg; tpl38Gt			2 figures from 2 publications
tpl2Tg; tpl39Gt			2 figures from 2 publications
tpl2Tg; tpl40Gt			2 figures from 2 publications
tpl2Tg; tpl41Gt			2 figures from 2 publications
tpl2Tg; tpl42Gt			2 figures from 2 publications
tpl2Tg; tpl43Gt			2 figures from 2 publications
triqk^tpl21Gt/+; tpl2Tg/+ (AB)	triqk
triqk^tpl21Gt; tpl2Tg	triqk		2 figures from 2 publications
zfp36l1a^tpl8Gt/+; tpl2Tg/+ (AB)	zfp36l1a
zfp36l1a^tpl8Gt; tpl2Tg	zfp36l1a		2 figures from 2 publications

Sequence Information

Other Construct Pages

None

Citations