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aip WT and mutant transcript counts in five dpf RNA-seq samples. Sample genotype and line are presented on the x-axis, and transcript counts from the integrated genome viewer (IGV) web app are on the y-axis. Transcript type is denoted by color. Individual sample counts correspond to the black circles overlayed on the bars. Significant differences between genotype are denoted by an asterisk, * (p-value ≤0.05, Kruskal–Wallis test followed by Dunn’s post hoc tests with Holm correction, error bars = standard error). Statistically significant differences in transcript abundance could only be found within line and between aip+/+and aip−/− larvae. However, it appears that aip+/− larvae have an intermediate number of WT transcripts and that mutant transcripts are largely degraded, likely through nonsense mediated decay. aip−/− larvae have some maternal WT transcripts, but very few aip transcripts overall.
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