Fig. 5

In silico analysis of dut expression during zebrafish embryogenesis and germ cell development. (A, B) Expression levels of dut transcripts and dUTPase protein shown as boxplot reflecting the median, interquartile range (IQR), and whiskers representing 1.5 × IQR compared to normalized means (blue) around the MTZ (3 hpf). Graph is created by Shiny script described in ref [63]. (TPM stands for “Transcripts per million”; protein abundance reflects normalized values of mass spectrometry measurements of three (Panel A) or four (Panel B) biological replicates; all data from: [63]). (C) GO analysis of genes that are significantly coexpressed with dut from 4 hpf to 120 hpf. (Based on data from: [64]). GeneRatio represents the proportion of genes that are coexpressed with dut and are annotated to a specific GO term, whereas Count represents the raw number of genes annotated to the GO term. The p.adjust value reflects the adjusted p value for the enrichment, using the Benjamini–Hochberg adjustment method. (D, E) Expression of dut during various stages of oogenesis (D) and spermatogenesis (E). (Based on data from: [67, 68].) The percentage of cells in the dataset expressing the gene (% expr) is shown, as is the average expression level (Avg. expr.) of the gene across all the cells in a given subgroup, calculated based on the normalized expression of the genes in the dataset.