Fig. 2

Spatial arrangement of Danio rerio dUTPase mutations relative to the surface and the active site of the protein. (A, B) Surface of DrDUT‐RGV (Alphafold 3 model) is shown (partially transparent) from two different viewpoints (rotated around the y axis by 90 degrees) to allow inspection of all three positions. Protein chains are colored yellow, green and cyan, positions of the variable residues in case of Chain A (yellow) highlighted with magenta. The position of the substrate analogue (shown as sticks with atomic coloring carbon : black, oxygen : red, nitrogen : blue, phosphorus : orange) at the active site is modeled based on superimposition of DrDUT‐RGV with the human dUTPase (PDB ID: 3EHW). (C) Intrachain hydrogen bonds of D92 with residues Y77, N131, and T55. The extended part of the carboxy terminus is not shown. Figure was created using pymol 2.5.4 (Schrodinger, LLC; https://www.pymol.org).