FIGURE

Fig. 3

ID

ZDB-FIG-260528-24

Publication

Martinez-Mayer et al., 2026 - Generation of a prop1 knockin zebrafish enables single-cell transcriptomics of early pituitary development

Other Figures

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Fig. 3

scRNA-seq of the early teleost pituitary reveals epcam as a conserved adenohypophyseal marker. (A) Schematic view of the scRNA-Seq experiment: (1) Dissection, (2) single-cell suspension, (3) FACS, and (4) 10× sequencing. (B) UMAP showing both embryonic stages. (C) UMAP representation according to Leiden clustering. (D and E) mScarlet and epcam expression levels. (F) Validation of epcam expression in the adenohypophysis using FISH at 28 and 52 hpf, in prop1:GAL4;UAS:mScarlet-CAAX zebrafish embryos. Maximum intensity projection of immunofluorescence against mScarlet (purple) and FISH against epcam (cyan). (G) In situ hybridization against Epcam in an E14.5 embryo, from the GenePaint database (19) (Epcam—ST62-EX132.8.3.C). (H) Immunofluorescence against EPCAM in Wylde type mouse embryos (E11.5, E12.5, E14.5 and P7) and Prop1−/− at E14.5. Co-immunofluorescence against Lhx3 (green) and Epcam (red) in a wild-type mouse at E12.5 (bottom panel, middle).

Expression Data

Gene:	epcam
Fish:	prop1^bns714Tg/+; bns681Tg (AB)
Anatomical Terms:	adenohypophysis hypophysis oral epithelium
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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