FIGURE

Fig. 5

ID

ZDB-FIG-260521-32

Publication

Nayak et al., 2025 - Dynein axonemal assembly factors (dnaaf) 5 and 9 are expressed in ciliated organs of zebrafish embryos

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

dnaaf9 crispants exhibit scoliotic-like phenotype. >(A) Illustration of the dnaaf9 gene structure, showing exons (boxes), introns (lines) and exons targeted by guide RNAs (blue boxes) along with the primer positions used for genotyping (Orange arrowhead-Exon 6, Green arrow-Exon 30). (B,C) Heteroduplex assay result confirming high efficiency of guide RNA–mediated mutagenesis in F0 crispants. (D) Representative morphological images of 6 dpf dnaaf9 crispants exhibiting notochord malformation (red arrows). (E) Genotyping result showing efficient mutation in dnaaf9 crispants exhibiting notochord defect. (F) Representative sequencing results showing the position of the deletion of 24.7 kb between exons 6 and 30. Scale bars, 150 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU + CRISPR1-dnaaf9 + CRISPR2-dnaaf9
Knockdown Reagents:	CRISPR1-dnaaf9 CRISPR2-dnaaf9
Observed In:	notochord
Stage:	Day 6

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Dev. Biol.