FIGURE

Fig. 4

ID

ZDB-FIG-260521-31

Publication

Nayak et al., 2025 - Dynein axonemal assembly factors (dnaaf) 5 and 9 are expressed in ciliated organs of zebrafish embryos

Other Figures

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Fig. 4

dnaaf5 crispants exhibit primary ciliary dyskinesia (PCD)-like phenotypes. (A) Schematic representation of the dnaaf5 gene structure, showing exons (boxes), introns (lines) and the region targeted by gRNA (blue box) along with the position of the primers used for genotyping (Orange arrow). (B,C) Genotyping results demonstrating efficient mutagenesis in 12.5 and 25 ng/μL gRNA-injected crispants. (D) Representative morphological images of 4-day post fertilisation (dpf) dnaaf5 crispants exhibiting PCD-like phenotypes, including body curvature (square grouping brace), pericardial edema (asterisk), and glomerular cyst (circle) compared to WT. (E) Genotyping result showing efficient mutation in dnaaf5 crispants showing phenotype combination. Scale bars, 150 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU + CRISPR1-dnaaf5
Knockdown Reagent:	CRISPR1-dnaaf5
Observed In:	pericardium pronephric glomerulus whole organism anterior-posterior axis
Stage:	Day 4

Phenotype Detail

Acknowledgments

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