FIGURE

Fig. 1

ID
ZDB-FIG-260505-103
Publication
Li et al., 2026 - Screening and characterization of a novel cupin-like polypeptide from Oyster (Ostrea gigas) and its anti-inflammatory effect via TAK1 mediated NF-κB pathway
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Fig. 1

Purification and identification of polypeptide OGP1-1 from O. gigas. (A) Isolation of O. gigas polypeptide extract by a TSKgel G2000SWXL column. (B) Isolation of native OGP1-1 (green arrow) from the pooled polypeptide fraction by a C18 RP-HPLC column. (C) The purity profile of OGP1-1 detected by RP-HPLC. (D) Inhibition of NO production in LPS-stimulated RAW264.7 macrophages induced by P1-P4 fractions. Three independent experiments were conducted to calculate the mean ± SD (**P < 0.01 vs. the control group; #P < 0.05, ##P < 0.01 vs. LPS group). (E) Reducing and non-reducing SDS-PAGE profiles of OGP1-1, respectively. Lane 1, Marker, Lane 2, OGP1-1 reducing sample, Lane 3, Marker, Lane 4, OGP1-1 non-reducing sample. (F) Evaluation of cell viability of OGP1-1 in LPS-stimulated RAW264.7 macrophages. (G) Inhibition of NO production in LPS-stimulated RAW264.7 macrophages induced by OGP1-1. Three independent experiments were conducted to calculate the mean ± SD (**P < 0.01 vs. the control group; #P < 0.05, ##P < 0.01 vs. LPS group). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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