Fig. 1

Fig. 1

Purification and identification of polypeptide OGP1-1 from O. gigas. (A) Isolation of O. gigas polypeptide extract by a TSKgel G2000SWXL column. (B) Isolation of native OGP1-1 (green arrow) from the pooled polypeptide fraction by a C18 RP-HPLC column. (C) The purity profile of OGP1-1 detected by RP-HPLC. (D) Inhibition of NO production in LPS-stimulated RAW264.7 macrophages induced by P1-P4 fractions. Three independent experiments were conducted to calculate the mean ± SD (**P < 0.01 vs. the control group; #P < 0.05, ##P < 0.01 vs. LPS group). (E) Reducing and non-reducing SDS-PAGE profiles of OGP1-1, respectively. Lane 1, Marker, Lane 2, OGP1-1 reducing sample, Lane 3, Marker, Lane 4, OGP1-1 non-reducing sample. (F) Evaluation of cell viability of OGP1-1 in LPS-stimulated RAW264.7 macrophages. (G) Inhibition of NO production in LPS-stimulated RAW264.7 macrophages induced by OGP1-1. Three independent experiments were conducted to calculate the mean ± SD (**P < 0.01 vs. the control group; #P < 0.05, ##P < 0.01 vs. LPS group). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)