FIGURE

Fig. 6

ID

ZDB-FIG-260430-34

Publication

Baek et al., 2026 - A novel small molecule KMU-11361 attenuates rheumatoid arthritis by mechanistic inhibition of the TAK1-NF-κB-NLRP3 axis

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Fig. 6

Effect of KMU-11361 on the osteoblast in the respective differentiation of MC3T3. A ALP activity was assessed on day 7 using ALP staining, and mineralization was evaluated on day 14 through Alizarin Red staining. Representative well images of ALP staining and corresponding magnified views (top). Scale bar, 100 μm. Representative images of ARS-stained wells and mineralized nodules stained with Alizarin Red S (bottom). Scale bar, 200 μm. B Quantitative analysis of intracellular ALP activity in MC3T3-E1 cells cultured under osteoblast differentiation conditions with or without KMU-11361 (0.1, 0.25, and 0.5 µM) for 3 to 14 days. C MC3TC-E1 cells were pretreated with compound KMU-11361 (0.1, 0.25, and 0.5 µM) for 3 h, then subsequently cultured under osteoblast differentiation conditions for 3, 7, and 14 days. Total RNA was extracted and used to evaluate the mRNA expression levels of RUNX2, OPN, OCN, and ALP, respectively. *p < 0.05, **p < 0.01, and ***p < 0.001. D MC3TC-E1 cells were pretreated with compound KMU-11361 (0.1, 0.25, and 0.5 µM) for 3 h, then subsequently cultured under osteoblast differentiation conditions for 3 day. Whole-cell lysates were isolated and used to measure the protein expression levels of RUNX2 and OPN by western blotting

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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