FIGURE

Figure 6.

ID

ZDB-FIG-260427-54

Publication

Gao et al., 2026 - PCYT1A Hypophosphorylation Underlies Retinal Lipid Dysregulation in CERKL Retinitis Pigmentosa and Is Therapeutically Reversed by Phosphatidylcholine

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Figure 6.

Depletion of CERKL decreased serine phosphorylation of PCYT1A. (A) Workflow of the phosphoproteomic analysis from WT and cerkl^−/− zebrafish retinas. (B) Protein domain analysis for phosphoproteins with changed phosphorylation level. (C) Quantitative analysis of Pcyt1aa sites relative phosphorylation level. (D) Alignment of amino acid sequence of PCYT1A between human and zebrafish. (E) Nile red staining in RPE-1 cells after NC and siCERKL transfection with flag-tagged PCYT1A or PCYT1A mutants. Scale bars = 10 µm. (F) Quantitative analysis of the cells is shown in E. At least 30 cells were quantified per group. (G) Nile red staining in RPE-1 cells after NC and siCERKL transfection with flag-tagged PCYT1A or PCYT1A mutants. Scale bars = 10 µm. (H) Quantitative analysis of the cells is shown in G. At least 30 cells were quantified per group. The results are shown as mean ± SD. ***, P < 0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cerkl^hzu3/hzu3
Observed In:	retina CDP-choline pathway
Stage:	Adult

Phenotype Detail

Acknowledgments

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