ZFIN Image: Gao et al., 2026, Figure 6.

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Figure Caption

Figure 6.

Depletion of CERKL decreased serine phosphorylation of PCYT1A. (A) Workflow of the phosphoproteomic analysis from WT and cerkl^−/− zebrafish retinas. (B) Protein domain analysis for phosphoproteins with changed phosphorylation level. (C) Quantitative analysis of Pcyt1aa sites relative phosphorylation level. (D) Alignment of amino acid sequence of PCYT1A between human and zebrafish. (E) Nile red staining in RPE-1 cells after NC and siCERKL transfection with flag-tagged PCYT1A or PCYT1A mutants. Scale bars = 10 µm. (F) Quantitative analysis of the cells is shown in E. At least 30 cells were quantified per group. (G) Nile red staining in RPE-1 cells after NC and siCERKL transfection with flag-tagged PCYT1A or PCYT1A mutants. Scale bars = 10 µm. (H) Quantitative analysis of the cells is shown in G. At least 30 cells were quantified per group. The results are shown as mean ± SD. ***, P < 0.001.

Acknowledgments

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