Regulation of SHOX2 expression by MEIS2 O-GlcNAcylation. HEPM cells were subjected to transfection with OGT shRNA lentiviral vectors for inducing endogenous OGT knockdown and then infected with expressing MEIS2 (WT or S237A) plasmids. a Representative Immunofluorescence staining of MEIS2 in HEPM cells treated as described above. b Nuclear and cytosolic fractions underwent IB with anti-MEIS2, and the ratio of the nucleus to the cytoplasm in MEIS2 was quantified. n = 3 biologically independent experiments. c WB analysis of SHOX2 and MEIS2 protein levels in HEPM cells treated as described above. d Dual-luciferase reporter assays showed the effects of MEIS2 overexpression on relative SHOX2-promoter activity in HEPM cells. n = 3 biologically independent experiments. e The activity of SHOX2 promoter luciferase reporter constructs was measured by dual luciferase reporter assays. n = 3 biologically independent experiments. Data were indicated by mean ± SD. P-values were computed by one-way ANOVA followed by Tukey’s multiple comparisons test, *P < 0.05 and **P < 0.01
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