Fig. 4.

Sensitivity of AIP mutant zebrafish larvae to the developmental toxicity of dioxin-like compounds. A) PCB126 (experiment 4). Heterozygous aip^wh86 zebrafish were crossed and their embryos were exposed to DMSO (0.02%) or PCB126 (10 nM) from 6 to 24 hpf. At 72 hpf, the degree of edema was scored and the larvae were genotyped. Additional information about this experiment can be found in Fig. S8. B) TCDD (experiment 6). Heterozygous aip^wh86 zebrafish were crossed and their embryos were exposed in 96-well plates to DMSO (0.1%) or TCDD (1 or 10 nM) from 6 to 120 hpf. At 24 and 120 hpf, larvae were assessed for 15 morphological endpoints and then genotyped. To account for some background defects in the AIP mutants, a background correction was applied and the percent difference in defects relative to DMSO controls was determined. *P < 0.05 vs. DMSO (Fisher’s exact test). Additional information about this experiment can be found in Fig. S10. Abbreviations: TCDD: 2,3,7,8-tetrachlorodibenzo-p-dioxin; PCB126: 3,3′,4,4′,5-pentachlorobiphenyl; WT: Wild-type; HET: Heterozygous; HMZ: Homozygous.