Figure 5

Reactome pathway analysis of TBT-induced alterations in neurotransmitter systems. (A) Pathway enrichment analysis based on Reactome database, with neurotransmitter-related pathways highlighted in red text showing significant enrichment. The x-axis represents the count of genes involved in each pathway, while the color gradient indicates the statistical significance (−log10 (p-value)). (B) Hierarchical network analysis of TBT-affected neurotransmitter pathways. Upper panel: Network visualization showing significantly affected neurotransmitter pathways (green nodes) and their associated key genes and metabolites (red nodes). Lower panel: Detailed protein–protein interaction network displaying differentially expressed genes (red nodes with asterisks) and their interaction partners (blue nodes) within the neurotransmitter pathways. Different types of molecular interactions are indicated by distinct line styles as shown in the legend. The asterisk (*) denotes differentially expressed genes with p < 0.05. The gray notes represent non-significant genes.