FIGURE

Fig. 4.

ID

ZDB-FIG-260311-200

Publication

Aleman et al., 2026 - Crip2 preserves hematopoietic stem and progenitor cell production through inhibition of Notch signals

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Fig. 4.

crip^DM embryos exhibit upregulation of the vascular gene signature. (A-C) In situ hybridization (ISH) for cdh5 in wild-type (n=40) (A) and crip^DM (n=41) (B) embryos at 26 hpf. Lateral views, anterior to the left. Quantification of cdh5 signal in the dorsal aorta (DA) using pixel intensity analysis (C) shows enhanced signal in crip^DM embryos employing an unpaired nonparametric Mann–Whitney U-test (****P<0.0001). (D-F) ISH for fli1 in wild-type (n=56) (D) and crip^DM (n=57) (E) embryos at 26 hpf. Lateral views, anterior to the left. Quantification of fli1 signal in the DA using pixel intensity analysis (F). An unpaired two-tailed nonparametric t-test yields a statistically significant difference between wild-type and crip^DM (****P<0.0001) embryos. (G-I) ISH for efnb2a in wild-type (n=54) (G) and crip^DM (n=53) (H) embryos at 26 hpf. Lateral views, anterior to the left. Quantification of efnb2a signal in the DA using pixel intensity analysis (I). An unpaired two-tailed nonparametric t-test yields a statistically significant difference between wild-type and crip^DM (****P<0.0001) embryos. Mean and standard error of each dataset are shown. Scale bars: 100 µm.

Expression Data

Genes:	cdh5 efnb2a fli1
Fish:	crip2^fcu2/fcu2; crip3^fcu3/fcu3
Anatomical Terms:	artery endothelial cell
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	crip2^fcu2/fcu2; crip3^fcu3/fcu3
Observed In:	artery endothelial cell
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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