Fig. 4.

Fig. 4.

crip^DM embryos exhibit upregulation of the vascular gene signature. (A-C) In situ hybridization (ISH) for cdh5 in wild-type (n=40) (A) and crip^DM (n=41) (B) embryos at 26 hpf. Lateral views, anterior to the left. Quantification of cdh5 signal in the dorsal aorta (DA) using pixel intensity analysis (C) shows enhanced signal in crip^DM embryos employing an unpaired nonparametric Mann–Whitney U-test (****P<0.0001). (D-F) ISH for fli1 in wild-type (n=56) (D) and crip^DM (n=57) (E) embryos at 26 hpf. Lateral views, anterior to the left. Quantification of fli1 signal in the DA using pixel intensity analysis (F). An unpaired two-tailed nonparametric t-test yields a statistically significant difference between wild-type and crip^DM (****P<0.0001) embryos. (G-I) ISH for efnb2a in wild-type (n=54) (G) and crip^DM (n=53) (H) embryos at 26 hpf. Lateral views, anterior to the left. Quantification of efnb2a signal in the DA using pixel intensity analysis (I). An unpaired two-tailed nonparametric t-test yields a statistically significant difference between wild-type and crip^DM (****P<0.0001) embryos. Mean and standard error of each dataset are shown. Scale bars: 100 µm.