FIGURE

Figure 4

ID

ZDB-FIG-260215-32

Publication

Peng et al., 2026 - Estrogen Receptor 2b Is Involved in Regulating Gonadotropin-Inhibitory Hormone Expression During Early Development in Zebrafish

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Figure 4

β-estradiol treatment experiments. In the treatment experiments, each treatment group contains 30 embryos (3 parallel). All samples were treated from 48 hpf to 96 hpf. At 96 hpf, in situ hybridization, qPCR, and fluorescence signals were detected. (A) In situ hybridization to detect GnIH mRNA changes of wild-type zebrafish embryos treated with different doses of β-estradiol (1 μM, 10 μM, 50 μM). This treatment experiment was also repeated using transgenic zebrafish embryos. (B) In the wild-type zebrafish embryo treatment experiments, qPCR was also preformed to confirm the results. The results are presented as mean ± SEM values. Values accompanied by different letters are significantly different (ANOVA followed by Tukey’s test, p < 0.05).

Expression Data

Genes:	mCherry npvf
Fish:	WT zf4294Tg
Condition:	chemical treatment by environment: 17beta-estradiol
Anatomical Terms:	hypothalamus whole organism
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT zf4294Tg
Condition:	chemical treatment by environment: 17beta-estradiol
Observed In:	hypothalamus whole organism
Stage:	Day 4

Phenotype Detail

Acknowledgments

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