IMAGE

Figure 4

ID

ZDB-IMAGE-260215-42

Genes

mCherry
npvf

Publication

Peng et al., 2026 - Estrogen Receptor 2b Is Involved in Regulating Gonadotropin-Inhibitory Hormone Expression During Early Development in Zebrafish

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Figures for Peng et al., 2026

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Figure 4

β-estradiol treatment experiments. In the treatment experiments, each treatment group contains 30 embryos (3 parallel). All samples were treated from 48 hpf to 96 hpf. At 96 hpf, in situ hybridization, qPCR, and fluorescence signals were detected. (A) In situ hybridization to detect GnIH mRNA changes of wild-type zebrafish embryos treated with different doses of β-estradiol (1 μM, 10 μM, 50 μM). This treatment experiment was also repeated using transgenic zebrafish embryos. (B) In the wild-type zebrafish embryo treatment experiments, qPCR was also preformed to confirm the results. The results are presented as mean ± SEM values. Values accompanied by different letters are significantly different (ANOVA followed by Tukey’s test, p < 0.05).

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Animals (Basel)