FIGURE

Fig. 3

ID

ZDB-FIG-260210-162

Publication

Gao et al., 2026 - Mutations in VPS18 lead to a neutrophil maturation defect associated with disturbed vesicle homeostasis

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

VPS18 deficiency reduces CORVET and HOPS core complex subunit levels and leads to a disturbed vesicle homeostasis during neutrophil maturation.

A Schematic of CORVET and HOPS core (gray) and unique (green) subunits. B Expression levels of VPS11, VPS16 and VPS33A in indicated cell lines before (day 0) and after removal (day 2) of estrogen analyzed by mass spectrometry. C, E Expression levels of autophagy markers LC3B and p62 analyzed by mass spectrometry. D Representative Western blot of LC3B expression (left panel) and quantitative analysis (right panel) of LC3B-II expression in cell lysates from indicated cell lines during differentiation (day 0-4). Ratios of LC3B-II/GAPDH are presented as relative protein amount. n = 7. F Expression of p62 during differentiation (day 0-4) in indicated cell lines using flow cytometry. Relative p62 levels are shown (normalized to CTRL cells, 100%). n = 9. G Representative transmission electron microscopy images of CTRL, clone 1 and clone 2 dHoxb8 cells (left panel, scale bar, 2 µm). Squares, zoom-in areas shown in (a) (middle panel, scale bar, 500 nm) and (b) (right panel). Arrows, irregularly shaped vesicular structures. Arrowheads, autophagosomes. N, nucleus. H Quantification of horizontal (H) and perpendicular (P) size of vesicular structures and autophagosomes from (G). n = 1 independent experiment with n = 5 cells (CTRL & clone 1) and n = 3 cells (clone 2). I Expression levels of lysosomal marker LAMP1 analyzed by mass spectrometry. J Quantification of LysoTracker-stained lysosomes in indicated cell lines at day 4. n ≥ 13. Mean ± SD. **P < 0.01, ****P < 0.0001 compared to CTRL. ^####P < 0.0001 compared to Clone 1. One-way ANOVA, Tukey’s multiple comparisons test. B, C, E, I Mean ± SEM. n ≥ 2. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 compared to CTRL. One-way ANOVA, Dunnett’s multiple comparison test. ns, not significantly different. D, F Mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 compared to CTRL. Two-way ANOVA, Tukey’s multiple comparisons test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Death Dis.