ap2m1a is crucial for hepatocyte proliferation in zebrafish embryo model. A The structure of human AP2M1 (top) and zebrafish ap2m1a (bottom). The black arrow indicates structural differences between the two. B The amino acid sequence of human AP2M1 and zebrafish ap2m1a. The MHD (Mu homology domain) was represented by red box. C The expression of ap2m1a mRNA was detected by RT-qPCR in various concentration of ap2m1a-MO. Data were quantified and expressed as the mean ± SEM of 3 independent experiments. ***p < 0.001 vs. value in the negative control. D The expression of fabp10a mRNA was detected by RT-PCR in uninjected embryos and ap2m1a-MO 2.5 ng/nl injected embryos. Data were quantified and expressed as the mean ± SEM of 3 independent experiments. ***p < 0.001 vs. value in the negative control. E Quantitative analysis of the sizes of 27 uninjected embryos and 26 ap2m1a-MO 2.5 ng/nl injected embryos. fabp10a expression in those embryos was detected by WISH. The black arrow demonstrates zebrafish hepatocytes. Data were quantified and expressed as the mean ± SEM of each embryo. ****p < 0.0001 vs. value in the negative control
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