Fig. 6

Bony ray differentiation was blocked by DMH1 treatment. When stained with Milligan's Trichrome, sections of the regenerating fin of BRE510 fish treated with DMSO from 6dpa to 12dpa showed no evidence of Aniline blue-stained bone matrix near the tip (A), similar to DMH1-treated fish (B). About 130 μm away from the regenerating fin tip, slight Aniline blue staining was evident in developing bony rays of DMSO-treated fish (C, red arrows), but not in DMH1-treated fish (D). About 290 μm from the regenerating fin tip, moderate Aniline blue-stained bone matrix was evident in rays of DMSO-treated (E, red arrows), while slight Aniline blue staining of bone matrix was observed in DMH1-treated fish (F, red arrows). Strong Aniline blue-stained bone matrix was evident in rays about 600 μm from the regenerating fin tip in both DMSO-treated (G, red arrow) and DMH1-treated fish (H, red arrow). Panels A′-H′ are higher-magnification views of regions identified by the red dashed boxes in panels A–H, respectively. n = 3 for each chemical treatment. Scale bars: A-H = 500 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)