Smc3-KD leads to decreased kinociliary axoneme length in the first neuromast along the posterior lateral line, which is rescued by smc3 mRNA overexpression. (A) Representative images of the first posterior lateral line kinocilium in SC-KD (left), Smc3-KD (right). Larvae were fixed at 3 dpf. Acetylated tubulin is labeled in red, and DAPI is in blue. Arrows identify the base and tip of each measured kinocilium. Scale bar: 10.4 µm in the x and y dimensions. (B) Quantification of the decrease in axoneme length in Smc3-KD (Student's t-test, ****P<0.0001, SC-KD n=35, Smc3-KD=26). (C) Representative images of the first posterior lateral line kinocilium in SC-KD+smc3 mRNA (left), and Smc3-KD+smc3 mRNA (right). Scale bar: 10.4 µm. (D) smc3 mRNA co-injection in Smc3-KD rescues axoneme length as compared Smc3-KD alone (Student's t-test, **P=0.0123, Smc3-KD+smc3 mRNA n=26, Smc3-KD n=26) smc3 mRNA co-injection in SC-KD decreases axoneme length as compared SC-KD alone (Student's t-test, *P=0.0228, SC-KD+smc3 mRNA n=19, SC-KD n=35).
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