FIGURE

Figure 3.

ID

ZDB-FIG-251219-50

Publication

Stonkus et al., 2025 - A new role for PHYHD1 and related dioxygenases: demethylation of 2'-O-methylated nucleosides

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Figure 3.

Phytanoyl-CoA dioxygenase family demethylation activity was demonstrated by complementation of the uridine/cytidine auxotrophic E. coli HMS174(DE3) ΔpyrF strain after 1 week of growth at room temperature. Tested dioxygenases were expressed from either pET-21 or pLATE31 vectors. The abbreviated names of the oxygenases are indicated above the respective colonies. M9 medium was supplemented with 10 mg/l 2′-O-methyluridine.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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