Fig. 3

Sfrp1a affects Wnt signaling in the developing endothelium. A. Representative images of 7XTCF:GFP; kdrl:mCherryNLS fish injected with scramble MO or sfrp1a ATG MO and the double positive cells were analyzed for GFP fluorescence at 16.5 hpf. Arrow heads point to double positive endothelial cells in the floor of the dorsal aorta. Scale bars = 40 μm and inset is 10 μm. B. Quantification of GFP intensity in endothelial cells (n = 23 and 20 cells in 9 fish from each condition). Two-tailed student's, ∗p < 0.05. C. Representative flow cytometry histogram of 7XTCF:GFP; kdrl:mCherryNLS fish injected with scramble MO or sfrp1a ATG MO and the double positive cells were analyzed for GFP fluorescence at 16.5 hpf. Similar trends we observed in 2 additional trials. D. Flow cytometry quantification of GFP intensity in double positive cells (n = 20 fish per biological condition, 3 trials). Two-tailed student's, ∗p < 0.05. E. Representative images of 7XTCF:GFP; kdrl:mCherryNLS fish injected with 500 pg control mRNA or 500 pg sfrp1a mRNA and the double positive cells were analyzed for GFP fluorescence at 16.5 hpf. Arrow heads point to double positive cells in the floor of the dorsal aorta. F. Quantification of GFP intensity in floor of the dorsal aorta (n = 13 and 16 cells in 7 fish). Data were found to be non-normally distributed by Shapiro-Wilk test. Mann-Whitney U test, ∗∗p < 0.01. G. Representative flow cytometry histogram of 7XTCF:GFP; kdrl:mCherryNLS fish injected with 500 pg control mRNA or 500 pg sfrp1a mRNA and the double positive cells were analyzed for GFP fluorescence at 16.5 hpf. Similar trends we observed in 2 additional trials. H. Flow cytometry quantification of GFP intensity in double positive cells (n = 20 fish per biological condition, 3 trials). Two-tailed student's, ∗p < 0.05.