JMY aggravates PCSK9 loss‐induced NTDs in zebrafish. A) Detailed base diagram of the second exon region of zebrafish designed using PCSK9‐MO, resulting in 25 bp depletion. B) Effects of PCSK9‐MO, JMY‐mRNA, and their combined administration on the development of the zebrafish NTs. The left side shows the light microscopy image, and the right side shows a cross‐sectional HE‐stained image and an enlarged NT structure image. “*” indicates the neural cavity; scale bar, 1 mm (left) and 50 µm (right). C) RT‐PCR detection of PCSK9 expression after PCSK9‐MO administration. D) Percentage of incidence and mortality of zebrafish NTDs in the PCSK9‐MO, JMY‐mRNA, and combined treatment groups; n = 100 zebrafishes per group. E) RT‐PCR detection of JMY expression after JMY mRNA administration. F) Thermal images of zebrafish activities in the PCSK9‐MO, JMY‐mRNA, and combined administration groups. The right side shows the control group, and the left side shows the deformity group. G) Statistical analysis of zebrafish‐related behaviors in the PCSK9‐MO, JMY‐mRNA, and combined administration groups. The first group shows the distance of movement, the second group shows time, the third group shows speed, and the fourth group shows acceleration; n = 6 individual experiments. Values were mean ± SD. Statistical significance was determined using one‐way ANOVA with the Dunnet post hoc test (G); ns: P > 0.05.
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