FIGURE

Figure 3.

ID

ZDB-FIG-251105-32

Publication

Hall et al., 2025 - Comparison of the Differing Impacts of Lowered N-Acetylglucosaminyltransferase-Ia/b Activity on Motor and Sensory Function in Zebrafish

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Figure 3.

Comparison of oligomannosylated protein in mutant and Wt AB fish strains. Lectin blot and Coomassie blue-stained gels of embryo (48 hpf) (A), and tissues from adult strains (C–J). Quantification of the oligomannosylated proteins from embryos (n = 5; Panel (B)), and adult tissues, including swim bladder (SB, n = 3), skin (S, n = 3), heart (H, n = 3), skeletal muscle (SM, n = 3), spinal cord (SC, n = 3), brain (B, n = 4), brain regions 1 (R1, n = 5) and 2 (R2, n = 3) (Panel (K)). Brain regions 1 includes olfactory bulb, telencephalon, habenula, and about half of optic tectum, while brain region 2 includes about half of the optic tectum, cerebellum, and medulla. Numbers adjacent to the blots signify protein markers (in kDa). Data were compared Student’s t-test (* p at 0.02).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mgat1a^{ecu301/ecu301} (AB) mgat1b^{ecu300/ecu300} (AB)
Observed In:	brain protein N-acetylglucosaminyltransferase activity heart protein N-acetylglucosaminyltransferase activity integument protein N-acetylglucosaminyltransferase activity skeletal muscle protein N-acetylglucosaminyltransferase activity spinal cord protein N-acetylglucosaminyltransferase activity swim bladder protein N-acetylglucosaminyltransferase activity whole organism protein N-acetylglucosaminyltransferase activity
Stage Range:	Long-pec to Adult

Phenotype Detail

Acknowledgments

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