Figure 7.

Anxiety-type behavior was aberrant in adult mgat1 mutant fish. Computations of the time taken for fish to swim to the upper chamber of the tank (latency), the total number of times the fish moved from the lower to upper chamber, and the amount of time spent in the upper chamber were all measured in efforts to fully examine the impact of anxiety-type behavior in zebrafish. Data show results for each fish and the mean ± SEM for each measured parameter (n = 28, Wt AB; n = 18, mgat1b−/−; and n = 19, mgat1a−/−) (A) and (n = 33, Wt AB; n = 20, mgat1b−/−/; and n = 20, mgat1a−/−) (B,C). Data were compared using one-way Anova, along with Bonholm mean comparison test (* p < 0.05). Cohen’s d values signified medium to large differences between the three groups, as follows: WtAB vs. mgat1b, 0.76, 0.71, 0.73; Wt AB vs. mgat1a, 0.94, 0.40, 0.71; mgat1a vs. mgat1b, 1.68, 1.31, 0.1.46 for latency period, upper chamber number, and time in upper chamber, (B), and (C), respectively.