PA-supplementation leads to increase in body length, body weight and lipid accumulation in liver. (A) Schematic representation of the PA-supplementation paradigm. (B) Increased body length of test group upon PA-supplementation in comparison to control group. (C) Assessment of body weight of adult zebrafish fed with control diet or PA supplemented diet (n = 24). (D) Assessment of Liver-to-body weight ratio (n = 10). (E) Hematoxylin and Eosin staining of adult control and PA-fed 4 μm thick paraffin embedded liver tissue sections show increased accumulation of lipid droplets. (F) Oil Red O staining of adult control and PA-fed 8 μm thick liver cryosections (40 × magnification, images captured using DEBRO DIM 150, inverted microscope). Scale Bar: 50 μm. (G) Quantification of Oil Red O staining of control and PA-fed sections (quantified using ImageJ). (H) BODIPY staining of adult control and PA-fed 5 μm thick liver cryosections (63 × magnification, images captured using Confocal Leica DMi microscope). Scale Bar: 50 μm (I) Quantification of BODIPY staining of control and PA-fed sections (quantified using ImageJ).
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