Fig. 1

Calcium imaging of RSNs during behavior (A) Schematic of 2-photon calcium imaging and concurrent behavioral tracking. A subset of the sensory stimuli are illustrated and insets show eye and tail tracking. (B) Behavioral data for an example 30 s epoch during which a prey-like moving spot was presented (shaded box). Bottom images detail 2 swim bouts showing tail skeletons (time runs orange purple) and time-varying cumulative tail bend angle (black red corresponds to curvature along progressively more distal lengths of the tail). Swim 1048 was coincident with a convergent saccade as the animal initiated a hunting response toward the prey-like stimulus. (C) u508:GCaMP6f expression in the mid/hindbrain. Maximum intensity projection of image volume. A, anterior; P, posterior. (D) Schematic of RSN array (full labeling in Figure S1). (E) Calcium time series (blue) and OASIS-inferred spiking process (yellow, inverted for visualization clarity) for 3 example neurons. Boxed periods shown at expanded time scale on right. Purple triangles indicate times of swim bouts.