Fig. 7

Constitutively activated pERK in ppp2caa−/− manifests deregulated nutrient response. (A) pERK is activated by nutrients. Shown are representative western blots of pErk1/2, Erk1/2 and Gapdh from 3 months old zebrafish hearts and quantification of pErk and Erk. t-test was used to compare 2 groups. Data are presented as mean ± SEM, three biological replicates, **p < 0.01, ns, non-significant. (B) and (C) Representative images of pS6 and pErk in cryosectioned heart tissues of WT, dA/+, mek−/−, erk−/−, ppp1r1+/− and ppp2caa−/− zebrafish mutants at 0/4 h post feeding, co-immunostained using anti-α-actinin antibody with anti-pErk antibody or pS6 antibody. α-actinin, green. pERK and pS6, red. DAPI, blue. The open arrowheads and filled arrowheads indicate pErk and pS6, respectively. pErk, phosphorylated Erk1/2; pS6, phosphorylated S6 ribosomal protein. Scale bars: 20 μm. (D) Quantification of pErk and pS6 in (B) and (C). Data are presented as mean ± SEM, and one-way ANOVA was used to compare multiple groups, three biological replicates, **p < 0.01, WT, wild type, ns, non-significant.