Fig. 6

ppp1r10, but not ppp2caa inhibition, rescues the deregulated nutrient response in dA/+. Representative images and quantification of pS6 in cryosectioned heart tissues of WT, dA/+, ppp2caa+/−; dA/+ and ppp1r10+/−; dA/+ zebrafish mutants at 0/4 h post feeding, co-immunostained using anti-pS6 antibody and anti-α-actinin antibody. a-α−ctinin, green. pS6, red. DAPI, blue. The open arrowheads indicate pS6 signal. pS6 indicates phosphorylated S6 ribosomal protein. Scale bars: 20 μm. One-way ANOVA was used to compare multiple groups. Data are presented as mean ± SEM, three biological replicates, **p < 0.01. WT, wild type, ns, non-significant. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)