Fig. 9

HFD interacted with Myt1l mutation to influence weight with unchanged metabolic markers and feeding performance. (A) Following 14 weeks of HFD, Het mice weighed significantly more than WT littermates at P120. (B–E) HFD-fed Het mice did not differ in levels of fasted glucose (B), cholesterol (C), triglycerides (D) or free fatty acids (E) from HFD-fed WT mice. (F) Schematic of experimental timeline that included blood draw for metabolic marker assessment and feeding assay following 14 weeks of HFD. (G) During magazine training, Het and WT mice consumed a comparable number of pellets. (H–I) During dual active training, Het and WT mice consumed a comparable number of pellets (H) and exhibited a similar number of nose pokes (I). (J–K) During FR1 training, Het mice poked into the correct hole at a comparable rate to WT mice (I), resulting in a similar number of pellets consumed between groups (K). (L–M) During resetting PR testing, Het mice consumed a comparable number of pellets as WT mice (L) and exhibited a comparable number of correct pokes (M). Sample size for metabolic marker assessment, magazine, dual active and FR1 feeding assessments in panel A. Sample size for PR testing in panel L. Data are represented as means ± SEM with individual points presented as open circles. ANOVA (A–E) and repeated measures ANOVA (G–M) used for statistical comparisons.