Fig. 4

MMP-2/9 inhibition decreases the abundance and amplitude of SWs while enriching the percentage of longer duration SW events in early juvenile zebrafish. A Top: Experimental timeline for SB-3CT drug exposure and LFP recordings. Bottom: representative cartoon image of electrode placement in the ADL of ex vivo whole brain preparations. B Representative traces of SWR events from early juvenile zebrafish treated and not treated with SB-3CT (left) and late juvenile/early adults (right). LFP recordings (1–1000 Hz), filtered SW events (1–30 Hz), filtered ripple events (120–220 Hz), and frequency spectrogram. C–E Quantification of SW abundance, amplitude, and duration between DMSO- and SB-3CT-treated early juvenile zebrafish. F–H Quantification of SW abundance, amplitude, and duration between DMSO- and SB-3CT-treated late juvenile/early adult zebrafish. Mann-Whitney and Unpaired t tests. I Frequency distribution of SW duration for early juvenile zebrafish and K for late juvenile/early adult zebrafish between groups. Duration clusters are color-coded for identification: light gray represents cluster 1 (50–250 ms), light blue represents cluster 2 (300–600 ms), light red represents cluster 3 (650–1050 ms), and dark gray represents cluster 4 (1075–1600 ms). Solid lines correspond to the mean and the shaded area shows the S.E.M. The purple arrow shows a shift to the left in the frequency distribution of SW events from treated zebrafish whereas the red arrow shows a shift to the right. 2way ANOVA with Šídák’s multiple comparisons test (# represents significance). J, L Quantification of the percentage of SW events within a specified cluster and their change after MMP-2/9 inhibition. Graphs are expressed as mean ± S.E.M. *p < 0.05, ***p < 0.001, ****p < 0.0001. Created in BioRender. https://BioRender.com/y371lgm.