Fig. 6
- ID
- ZDB-FIG-250807-37
- Publication
- Wang et al., 2025 - EPHA4 signaling dysregulation links abnormal locomotion and the development of idiopathic scoliosis
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Aberrant swimming as a result of abnormal extension of cerebrospinal fluid-contacting neurons (CSF-cNs) axons. (A) Fluorescent images showing the distribution of ascending axons of CSF-cNs marked by Tg(pkd2l1:GAL4,UAS:Kaede) in 2 days post-fertilization (dpf) epha4a mutant larvae. Yellow line indicates the midline, and the red arrows indicate aberrantly extended axons in epha4a+/- and epha4a-/- larvae. (B) Bar graph showing the percentages of abnormal extension of CSF-cNs axons in 2 dpf wild-type, epha4a heterozygote, and epha4a homozygous. The numbers of larvae are indicated in each column. (C, C’) Superimposed frames of tail oscillations in 5 dpf control and epha4a morphants. (D, D’) A plot of body curvature angles in panels (C) and (C’). The positive angle means turning right. (E) Percentages of tendentious swing in control (N=8 larvae, n=24 experiments) and epha4a morphants (N=11 larvae, n=33 experiments). The percentages were calculated by the ratio of tendentious tail oscillation during the first eight swings. Scale bars: 50 µm in panel (A). |