FIGURE

Fig. 4

ID

ZDB-FIG-250807-19

Publication

Cao et al., 2025 - Spatiotemporal Super-Resolution Imaging of Lipid Metabolism Dynamics in Physiological/Pathological Conditions

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Fig. 4

a) STED-FLIM imaging (top-left) and its corresponding photon count image (bottom-right) of LDs in HeLa cells by STED-FLIM nanoscopy. Scale bar: 5 µm. b) STED and CLSM imaging in the field of view of ROI 7 in (a) and Figure S31. λex = 488 nm, λem = 650–725 nm, and λSTED = 775 nm. c) Zoomed-in map of (a) and Figure S31 in ROI 8. d) STED-FLIM image of LDs, the magnified image of the white dotted square is shown in Figure 5c. e) Fluorescence lifetime changes recorded along the white dotted line in (d). f) Fluorescence lifetime spectrums are recorded corresponding to positions (1, 2, 3, 4, and 5) in (d). ROS scavenger UA (50 µM) was added to avoid probe-induced lipid oxidation under light conditions.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Angew. Chem. Int. Ed. Engl.