FIGURE

Fig. 4

ID

ZDB-FIG-250730-84

Publication

Ingham et al., 2025 - Loss of SLX4IP leads to Common Fragile Sites instability and compromises DNA interstrand crosslink repair in vivo

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Fig. 4

SLX4IP-deficient U2OS cells exhibit chromosomal breakage and CFS underreplication. A, metaphase chromosome spreads of U2OS cells transfected with indicated siRNA. Gaps and breaks are indicated with arrows. B, quantification of chromosomal gaps and breaks as in (A) (mean ± SD; n = 2 biological replicates; >40 metaphase spreads analyzed per condition). C, representative images of EdU-positive chromosomes marking CFSs. D, quantification of (C) (mean ± SD; n = 2 biological replicates; >1800 chromosomes analyzed per condition). E, quantification of metaphase EdU foci (mean ± SD; n = 2 biological replicates; >600 chromosomes analyzed per condition). Cells were treated with 0,4 μM APH for 24h in all experiments. Significance was assessed by Student’s unpaired t-tests. Scale bar: 10 μM.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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