PUBLICATION
Loss of SLX4IP leads to Common Fragile Sites instability and compromises DNA interstrand crosslink repair in vivo
- Authors
- Ingham, A., Ramaswami, M., Ramangoudr-Bhojappa, R., Pladevall-Morera, D., De Santis, F., Terriente, J., Muñoz, I.M., Rouse, J., Chandrasekharappa, S.C., Lopez-Contreras, A.J.
- ID
- ZDB-PUB-250519-10
- Date
- 2025
- Source
- The Journal of biological chemistry : 110244110244 (Journal)
- Registered Authors
- Chandrasekharappa, Settara, De Santis, Flavia, Terriente, Javier
- Keywords
- none
- MeSH Terms
-
- Animals
- Chromosomal Instability*
- Chromosome Fragile Sites*
- DNA Repair*
- DNA Replication
- Fanconi Anemia/genetics
- Fanconi Anemia/metabolism
- Genomic Instability
- HeLa Cells
- Humans
- Recombinases*/genetics
- Recombinases*/metabolism
- Zebrafish/genetics
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- PubMed
- 40383148 Full text @ J. Biol. Chem.
Citation
Ingham, A., Ramaswami, M., Ramangoudr-Bhojappa, R., Pladevall-Morera, D., De Santis, F., Terriente, J., Muñoz, I.M., Rouse, J., Chandrasekharappa, S.C., Lopez-Contreras, A.J. (2025) Loss of SLX4IP leads to Common Fragile Sites instability and compromises DNA interstrand crosslink repair in vivo. The Journal of biological chemistry. :110244110244.
Abstract
Common Fragile Sites (CFSs) are chromosomal loci with inherent characteristics that make them difficult to fully replicate thus rendering them vulnerable to replication stress (RS). Under-replicated CFSs manifests as cytogenetic gaps and breaks on metaphase chromosomes. Moreover, CFSs are hotspots for tumorigenic chromosomal rearrangements. The Fanconi anemia (FA) pathway is at the core of a network of proteins that works to safeguard CFSs during replication and RS. Here, we uncover a novel role of SLX4IP in maintaining CFS stability. We show that SLX4IP localizes to stressed CFSs and that its loss exacerbates genome instability, including CFS expression. Furthermore, direct SLX4IP depletion leads to impaired replication and growth deficiencies. SLX4IP and FANCP/SLX4 are epistatic, suggesting that SLX4IP acts with SLX4 to maintain CFS stability. Finally, zebrafish larvae with homozygous knockout of slx4ip gene showed higher frequency of embryonic anomalies and sensitivity to DNA crosslinking agent, a typical cellular characteristic of FA patients. Our results establish a causal link between SLX4IP deficiency and chromosomal instability, which may explain how SLX4IP dysregulation contributes to cancer development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping