FIGURE

Fig. 5

ID

ZDB-FIG-250722-56

Publication

Fehilly et al., 2025 - Germline Disruption of Retinal Pigment Epithelium-Expressed Zebrafish rlbp1b^-/- Results in Selective Dim Light Visual Behavior Deficits and Provides a Screening Platform for Evaluating the Pathogenicity of Human RLBP1 Variants

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Fig. 5

rlbp1b knockout larvae have altered retinoid metabolism. (A) Schematic of experimental conditions where 50 whole larvae were collected at midday day 5 following standard raising conditions. Following this, retinoids were profiled by HPLC. All graphs represent three biological replicates for each sample. (B) Total retinoid levels are significantly reduced in rlbp1b knockout larvae (p = 0.0443, two tailed t-test). (C) 11-cis-retinal levels are significantly reduced in rlbp1b knockout larvae (p = 0.0015, two tailed t-test). (D) all-trans-retinal levels are significantly reduced in rlbp1b knockout larvae (p = 0.0263, two tailed t-test). (E, F) levels of less abundant 13-cis-retinal and 9-cis-retinal do not show any significant change in rlbp1b knockout larvae. (G, H) levels of retinal esters do not show any significant change in rlbp1b knockout larvae. (I) There is no significant change in levels of all-trans-retinol in rlbp1b knockout larvae. All graphs show mean ± standard deviation.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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