Fig 5

Recombinant mapping narrows region of interest to identify a loss-of-function allele of mttp.

A) markers A_a and B_a were outputted by WheresWalker and used to genotype arches mutants in order to identify recombinants. M and F denote male and female parents, respectively. PCR product sizes for wild-type and mutant (highlighted) products are as indicated. B) Points representing A_a (red) and B_a (blue) marker locations and horizontal lines representing the estimated distance to the causative mutation are overlaid on the SNP index for the arches interval (chromosome 1: 2.46-13.86 Mb). The vertical dashed line indicates the location of mttp, the causative locus. C) quantification of ApoBb.1-nanoluciferase levels at 3 dpf. Mean ± standard deviation, N = 4-5 clutches, n = 2-8 animals/datapoint. P < 0.05 by two-way ANOVA with Geisser-Greenhouse correction and Tukey’s multiple comparisons test. * vs. respective wild-type, ^ vs c655/ + , $ vs c655/c655, # vs stl/stl. D) Images of whole-animal ApoBb.1-nanoluciferase distribution in arches and stl mutants at 3 dpf. E) Brightfield images of mutant and wild-type animals from 2-4 dpf. For D and E, scale bar represents 1 mm.