Fig 2

Forward genetic screen identifies 28 dark yolk mutants.

A) Generation of mutant families using a standard forward genetic F3 screening scheme. B) Single locus hit rate for the slc45a2 locus was determined by crossing male founders to slc45a2^b4/b4 females and screening for albinism in the offspring; representative pigmented (pig.) and albino (alb.) 3 days post-fertilization (dpf) larvae are shown. C) Representative images of wild-type (wt) zebrafish from 3-6 dpf. D) Representative images of identified mutants; age, mutant name, and allele ID (cXXX) are noted. E) Screen mutants were crossed to known dark yolk mutants so that progeny could be evaluated for dark yolk. Representative images for 3 mutants that fail to complement known dark yolk loci. Phenotype frequency is reported as mean ± standard deviation. For arches: N = 4 clutches, n = 375 animals; For olympic: N = 5 clutches, n = 443 animals; For teton: N = 4 clutches, n = 396 animals. For all panels, scale bar represents 1 mm.