FIGURE

Fig. 5

ID

ZDB-FIG-250717-49

Publication

Castro-Sepulveda et al., 2025 - Protocol to measure spontaneous locomotion, graded exercise oxygen consumption, and cross-sectional area of skeletal muscle cells in zebrafish

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Fig. 5

Outline of the protocol to evaluate skeletal muscle fiber size in slow and fast fibers (A) Schematic representation of skeletal muscle dissection, cryoprotection and cryosection. The red square represents where the muscle sample is systematically selected. (B) Representative image of fast and slow fibers ROI positioning. The right panels show the zoom of the red ROI for each fiber type with the outline of selected fibers post thresholding. Automatically selected fibers are shown in colors, non-selected fibers and thresholding is shown in red. White crosses show examples of fibers that were manually excluded.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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