Fig. 2

bcas2 is required for hematopoietic progenitor differentiation. (A–C) Expression analysis of hemangioblast markers npas4l, scl, gata2 (A), erythroid progenitor markers gata1, hbbe3 (B), and myeloid marker pu.1 (C) in bcas2+/Δ14 embryos and their wild-type siblings at indicated stages. (D) Expression changes of gata1 in bcas2+/Δ14 embryos overexpressing BCAS2 at the 10-somite stage. The indicated embryos were injected with or without 300 pg of human BCAS2 mRNA at the one-cell stage. (E) Expression of endothelial marker fli1a in bcas2+/Δ14 and sibling embryos at the 10-somite stage. (F) Confocal imaging of bcas2+/Δ14 and control sibling Tg(kdrl:GFP) embryos at 54 hpf. Scale bars, 100 μm (A–E), 500 μm (F).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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