Fig. 5
- ID
- ZDB-FIG-250714-14
- Publication
- Ning et al., 2025 - BCAS2 promotes primitive hematopoiesis by sequestering β-catenin within the nucleus
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BCAS2 functions in CRM1-mediated nuclear export of β-catenin. (A) Tamoxifen-treated Bcas2-cKO mouse embryonic fibroblasts (MEFs) were incubated with 20 nM LMB for 3 h. The expression of Bcas2 and β-catenin was analyzed using immunofluorescence. The arrowheads show the cells with nuclear β-catenin accumulation. (B) SW480 cells were transfected with the indicated shRNA constructs and then treated with LMB for 3 h before immunostaining. GFP was regarded as a transfection control. The arrowheads indicate the transfected cells. (C, D) Immunofluorescence staining of β-catenin in bcas2 morphants with Tg(gata1:GFP) background at 16 hpf. Embryos were exposed to 20 nM LMB from the bud stage. The dotted lines indicate the GFP-positive hematopoietic progenitor cells. The relative fluorescence intensity of nuclear β-catenin was quantified in (D) (n=6). ns, not significant; **p<0.01 (Student’s t-test). (E) bcas2+/Δ14 embryos were treated with 20 nM LMB for 6 h and then subjected to WISH assay to analyze the expression of gata1 at the indicated stages. Scale bars, 10 μm (A, B), 5 μm (C), 100 μm (E). |