Fig. 1 - Supplemental 2
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- ZDB-FIG-250714-3
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- Ning et al., 2025 - BCAS2 promotes primitive hematopoiesis by sequestering β-catenin within the nucleus
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Zebrafish bcas2 mutants are generated by using CRISPR/Cas9 system. (A) Schematic showing generation of bcas2 mutants. Two mutant lines were obtained with mutations that resulted in premature translation termination, resulting in truncated Bcas2 proteins lacking the C-terminal CC domains. (B) Identification of bcas2 mutations using DNA sequencing. (C) The bcas2+/Δ7 and bcas2+/Δ14 mutants were identified via T7 endonuclease (the upper panel) or FspI restriction enzyme (the lower panel) digestions. (D) Bright-field images of embryos derived from crossing indicated females with heterozygous male mutants. Black arrows refer to the embryos that exhibited an abnormal cleavage. The ratio of viable embryos was indicated. Scale bars, 1 mm (D). |