Fig. 6

GABA receptor activity widely alters motor activity. (a) Hierarchical clustering of behavioral profiles across the 26-endpoint assay (x-axis) is presented for ten reference chemicals that target GABAA or GABAB receptors including agonists (yellow) and antagonists (purple). Heatmap colors, based on effect sizes (SSMD, strictly standardized median difference), indicate deviations from the median control phenotype. Red and blue hues represent increased and reduced motor activity, respectively (n = 32 larvae). (b) Multidimensional scaling (MDS) representation of pairwise distances between behavioral profiles, condensed across concentrations per chemical based on the LOEC (Lowest Observed Effect Concentration) shown in a. (c-h). Raincloud plots quantify the visual motor response under (c) illuminated, (d) non-illuminated (i.e., dark) conditions, (e-f) during a 1 sec acoustic startle response, (g) inter-acoustic endpoint intervals and (h) acoustic habituation sum for the GABAA receptor positive modulator Propofol, the GABAB receptor positive allosteric modulator CGP13501, and the GABAA receptor antagonists Bicuculline and Picrotoxin. Horizontal dotted lines indicate the median motor activity of vehicle-exposed larvae. Numbers above the rainclouds represent adjusted p-values (grey: p ≥ 0.05, black: p < 0.05, two-sample bootstrapping test with a Benjamin Hochberg post-hoc test, n (vehicle) = 72, n (treatment) = 36).